microglia morphology quantification tool Search Results


iba1 + microglia/macrophages  (Heindl GmbH)
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Heindl GmbH iba1 + microglia/macrophages
<t>IBA1</t> + areas transitioned to a more ameboid and swollen morphology after stroke in the ipsilateral hemisphere. Visual differences in density and morphological changes of IBA1 + areas were observed between NS and S animals (A,B) . Arrows indicate location of stroke lesion (B) . Significant ( p < 0.0001) increases in IBA1+ area occupied (C) , perimeter (D) , major axis length (E) , solidity ( F ; p = 0.0008), and mean radius (G) were observed in hemisphere section of S ( n = 1,860 ROIs) compared NS ( n = 1,362 ROIs). There was a significant ( p < 0.0001) decrease observed in eccentricity (H) of IBA1 + ROIs in hemisphere section analysis in S animals compared to NS animals. PCA (I) analysis showed significant ( p < 0.0001) increases in PC1 - PC3 (I–L) in S compared to NS animals. ** p < 0.001 and **** p < 0.0001.
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IBA1 + areas transitioned to a more ameboid and swollen morphology after stroke in the ipsilateral hemisphere. Visual differences in density and morphological changes of IBA1 + areas were observed between NS and S animals (A,B) . Arrows indicate location of stroke lesion (B) . Significant ( p < 0.0001) increases in IBA1+ area occupied (C) , perimeter (D) , major axis length (E) , solidity ( F ; p = 0.0008), and mean radius (G) were observed in hemisphere section of S ( n = 1,860 ROIs) compared NS ( n = 1,362 ROIs). There was a significant ( p < 0.0001) decrease observed in eccentricity (H) of IBA1 + ROIs in hemisphere section analysis in S animals compared to NS animals. PCA (I) analysis showed significant ( p < 0.0001) increases in PC1 - PC3 (I–L) in S compared to NS animals. ** p < 0.001 and **** p < 0.0001.

Journal: Frontiers in Cellular Neuroscience

Article Title: Semi-Automated Cell and Tissue Analyses Reveal Regionally Specific Morphological Alterations of Immune and Neural Cells in a Porcine Middle Cerebral Artery Occlusion Model of Stroke

doi: 10.3389/fncel.2020.600441

Figure Lengend Snippet: IBA1 + areas transitioned to a more ameboid and swollen morphology after stroke in the ipsilateral hemisphere. Visual differences in density and morphological changes of IBA1 + areas were observed between NS and S animals (A,B) . Arrows indicate location of stroke lesion (B) . Significant ( p < 0.0001) increases in IBA1+ area occupied (C) , perimeter (D) , major axis length (E) , solidity ( F ; p = 0.0008), and mean radius (G) were observed in hemisphere section of S ( n = 1,860 ROIs) compared NS ( n = 1,362 ROIs). There was a significant ( p < 0.0001) decrease observed in eccentricity (H) of IBA1 + ROIs in hemisphere section analysis in S animals compared to NS animals. PCA (I) analysis showed significant ( p < 0.0001) increases in PC1 - PC3 (I–L) in S compared to NS animals. ** p < 0.001 and **** p < 0.0001.

Article Snippet: For example, morphological changes of IBA1 + microglia/macrophages and GFAP + astrocytes following activation has been documented in many studies of neuroinflammation (Karperien et al., ; Hovens et al., ) and stroke specifically (Heindl et al., ).

Techniques:

Stroke resulted in an amoeboid morphological transition of IBA1 + areas in perilesional tissue. Significant ( p < 0.0001) increases in IBA1+ area occupied (A) , perimeter (B) , major axis length (C) , and mean radius (E) were observed in perilesional ROIs of S ( n = 452 ROIs) animals compared NS ( n = 304 ROIs) animals. There was a significant ( p < 0.0001) decrease in solidity (D) , and eccentricity (F) of IBA1+ perilesional ROIs in S animals compared to NS animals. PCA (G) analysis showed a significant ( p < 0.0001) increase in PC1-3 (H–J) in S compared to NS animals. **** p < 0.0001.

Journal: Frontiers in Cellular Neuroscience

Article Title: Semi-Automated Cell and Tissue Analyses Reveal Regionally Specific Morphological Alterations of Immune and Neural Cells in a Porcine Middle Cerebral Artery Occlusion Model of Stroke

doi: 10.3389/fncel.2020.600441

Figure Lengend Snippet: Stroke resulted in an amoeboid morphological transition of IBA1 + areas in perilesional tissue. Significant ( p < 0.0001) increases in IBA1+ area occupied (A) , perimeter (B) , major axis length (C) , and mean radius (E) were observed in perilesional ROIs of S ( n = 452 ROIs) animals compared NS ( n = 304 ROIs) animals. There was a significant ( p < 0.0001) decrease in solidity (D) , and eccentricity (F) of IBA1+ perilesional ROIs in S animals compared to NS animals. PCA (G) analysis showed a significant ( p < 0.0001) increase in PC1-3 (H–J) in S compared to NS animals. **** p < 0.0001.

Article Snippet: For example, morphological changes of IBA1 + microglia/macrophages and GFAP + astrocytes following activation has been documented in many studies of neuroinflammation (Karperien et al., ; Hovens et al., ) and stroke specifically (Heindl et al., ).

Techniques:

GFAP + areas transitioned to more sprawled and extended morphology after stroke in ipsilateral tissue. Visual differences in density and location of GFAP + areas were observed in ipsilateral sections, between S and NS animals (A,B) . Arrows indicate location of stroke lesion (B) . Significant ( p < 0.0001) increases in perimeter (D) , major axis length (E) , solidity ( F ; p = 0.0003), and mean radius (G) were observed in hemispheric section ROIs of S ( n = 1,848 ROIs) animals relative to NS ( n = 1,668 ROIs) animals. There was a significant ( p < 0.0001) decrease observed in GFAP + area occupied (C) and solidity (F) of IBA1 + ROIs of in S animals compared to NS animals. No significant difference was seen in eccentricity (H) . PCA (I) analysis revealed a significant ( p < 0.0001) difference in PC1-3 (J–L) in S animals compared to NS animals. **** p < 0.0001.

Journal: Frontiers in Cellular Neuroscience

Article Title: Semi-Automated Cell and Tissue Analyses Reveal Regionally Specific Morphological Alterations of Immune and Neural Cells in a Porcine Middle Cerebral Artery Occlusion Model of Stroke

doi: 10.3389/fncel.2020.600441

Figure Lengend Snippet: GFAP + areas transitioned to more sprawled and extended morphology after stroke in ipsilateral tissue. Visual differences in density and location of GFAP + areas were observed in ipsilateral sections, between S and NS animals (A,B) . Arrows indicate location of stroke lesion (B) . Significant ( p < 0.0001) increases in perimeter (D) , major axis length (E) , solidity ( F ; p = 0.0003), and mean radius (G) were observed in hemispheric section ROIs of S ( n = 1,848 ROIs) animals relative to NS ( n = 1,668 ROIs) animals. There was a significant ( p < 0.0001) decrease observed in GFAP + area occupied (C) and solidity (F) of IBA1 + ROIs of in S animals compared to NS animals. No significant difference was seen in eccentricity (H) . PCA (I) analysis revealed a significant ( p < 0.0001) difference in PC1-3 (J–L) in S animals compared to NS animals. **** p < 0.0001.

Article Snippet: For example, morphological changes of IBA1 + microglia/macrophages and GFAP + astrocytes following activation has been documented in many studies of neuroinflammation (Karperien et al., ; Hovens et al., ) and stroke specifically (Heindl et al., ).

Techniques:

Stroke resulted in larger A1-like morphological changes of GFAP + areas in perilesional tissue. Significant ( p < 0.0001) increases in GFAP + area occupied (A) , compactness (C) , and major axis length (E) were observed in ROIs of S ( n = 461 ROIs) animals compared NS ( n = 324 ROIs) animals. There was a significant decrease in form factor ( B ; p < 0.0001), solidity ( D ; p < 0.0001) and orientation ( F ; p = 0.0002) of IBA1 + ROIs in the perilesional region of S animals compared to NS animals. Upon PCA (G) analysis, a significant increase was observed in PC1 ( H ; p < 0.0001) and PC3 ( J ; p = 0.0162), but no significant difference was observed in PC2 (I) . * p < 0.05, *** p < 0.001, and *** p < 0.0001.

Journal: Frontiers in Cellular Neuroscience

Article Title: Semi-Automated Cell and Tissue Analyses Reveal Regionally Specific Morphological Alterations of Immune and Neural Cells in a Porcine Middle Cerebral Artery Occlusion Model of Stroke

doi: 10.3389/fncel.2020.600441

Figure Lengend Snippet: Stroke resulted in larger A1-like morphological changes of GFAP + areas in perilesional tissue. Significant ( p < 0.0001) increases in GFAP + area occupied (A) , compactness (C) , and major axis length (E) were observed in ROIs of S ( n = 461 ROIs) animals compared NS ( n = 324 ROIs) animals. There was a significant decrease in form factor ( B ; p < 0.0001), solidity ( D ; p < 0.0001) and orientation ( F ; p = 0.0002) of IBA1 + ROIs in the perilesional region of S animals compared to NS animals. Upon PCA (G) analysis, a significant increase was observed in PC1 ( H ; p < 0.0001) and PC3 ( J ; p = 0.0162), but no significant difference was observed in PC2 (I) . * p < 0.05, *** p < 0.001, and *** p < 0.0001.

Article Snippet: For example, morphological changes of IBA1 + microglia/macrophages and GFAP + astrocytes following activation has been documented in many studies of neuroinflammation (Karperien et al., ; Hovens et al., ) and stroke specifically (Heindl et al., ).

Techniques:

Stroke alters relationship of morphological parameters across cell types. PCA of NS and S ROIs labeled by DCX (red), FactorVIII (light green), GFAP (dark blue), IBA1 (tan), and NeuN (dark green). PCA ellipses for each stain are well-delineated in NS animals (A) , but not in S animals (B) . Heatmaps of each parameter measured 1-19 were generated for GFAP vs. IBA1 (C) , IBA1 vs. NeuN (D) , and NeuN vs. GFAP (E) for each treatment group (S and NS) as well as each ROI location (perilesional or hemispheric section). NS animals generally showed an increase in strength of correlations, positively or negatively, when comparing heatmaps of perilesional areas and hemispheric section areas. In S animals, there often was a complete reversal of correlation directionality between perilesional and hemispheric sections (C–E) . Linear correlations, representative of one square of the heatmap showed significant correlations of perilesional IBA1 extent and GFAP mean radius in S ( p = 0.0220) but not NS animals ( p = 0.6779) (F) ; in hemispheric section IBA1 major axis length and GFAP cell area in S animals ( p = 0.0289), but not NS animals ( p = 0.6402); in hemispheric section ROIs of GFAP eccentricity and IBA1 solidity in NS animals ( p = 0.0311), but not S animals ( p = 0.3103) (G) ; and in perilesional GFAP eccentricity and IBA1 solidity in S animals ( p = 0.0011), but not NS animals ( p = 0.7858) (H) .

Journal: Frontiers in Cellular Neuroscience

Article Title: Semi-Automated Cell and Tissue Analyses Reveal Regionally Specific Morphological Alterations of Immune and Neural Cells in a Porcine Middle Cerebral Artery Occlusion Model of Stroke

doi: 10.3389/fncel.2020.600441

Figure Lengend Snippet: Stroke alters relationship of morphological parameters across cell types. PCA of NS and S ROIs labeled by DCX (red), FactorVIII (light green), GFAP (dark blue), IBA1 (tan), and NeuN (dark green). PCA ellipses for each stain are well-delineated in NS animals (A) , but not in S animals (B) . Heatmaps of each parameter measured 1-19 were generated for GFAP vs. IBA1 (C) , IBA1 vs. NeuN (D) , and NeuN vs. GFAP (E) for each treatment group (S and NS) as well as each ROI location (perilesional or hemispheric section). NS animals generally showed an increase in strength of correlations, positively or negatively, when comparing heatmaps of perilesional areas and hemispheric section areas. In S animals, there often was a complete reversal of correlation directionality between perilesional and hemispheric sections (C–E) . Linear correlations, representative of one square of the heatmap showed significant correlations of perilesional IBA1 extent and GFAP mean radius in S ( p = 0.0220) but not NS animals ( p = 0.6779) (F) ; in hemispheric section IBA1 major axis length and GFAP cell area in S animals ( p = 0.0289), but not NS animals ( p = 0.6402); in hemispheric section ROIs of GFAP eccentricity and IBA1 solidity in NS animals ( p = 0.0311), but not S animals ( p = 0.3103) (G) ; and in perilesional GFAP eccentricity and IBA1 solidity in S animals ( p = 0.0011), but not NS animals ( p = 0.7858) (H) .

Article Snippet: For example, morphological changes of IBA1 + microglia/macrophages and GFAP + astrocytes following activation has been documented in many studies of neuroinflammation (Karperien et al., ; Hovens et al., ) and stroke specifically (Heindl et al., ).

Techniques: Labeling, Staining, Generated